Fig. 5

Rapid, robust, and cost-efficient diagnostics using findONTime in combination with MinION sequencing. A simulated scenario of hypothesis-free ONT sequencing using data from a MPXV-positive sample, prepared without prior viral enrichment/host depletion. The plot shows the number of reads mapping to a MPXV reference genome and the percentage of horizontal coverage at increasing time points, during the sequencing run. Reference genome identified with over 50% coverage after 20 min. Contigs mapped at the 40-min mark. Strong evidence (mapped contigs; > 90% reference genome covered by at least one read) is achieved in under 2 h (1 h 20 min)