Fig. 6

Correlation between deterministic genes and Hypo-MS4 activity. A FOXA1 binding landscape of Hypo-MS4-related DMPs in the gene body (n = 162) and TSS regions (n = 98) in the A549 and HepG2 cell lines. B FOXA1 binding landscape of Hypo-MS4-related DMPs in the gene body (n = 162) and TSS regions (n = 98) in colorectal and breast cancer tissues. C Correlation between the mean methylation level of FOXA1-binding sites and Hypo-MS4 activity in single-cell methylome data of colorectal cancer patients (left panel: FOXA1-binding sites in the TSS; right panel: FOXA1-binding sites in the gene body). D FOXA1 expression levels in patients with low and high Hypo-MS4 activity (according to the median Hypo-MS4 activity) in TP53-MUT and TP-53 WT tumors from LIHC, BRCA, and HNSC. E Hypo-MS4-GES scores increase in TP53-MUT and TP53-WT melanoma patients who are nonresponders to immune therapy