Fig. 5
m6A modification is associated with the circularization of circRNAs. A Distribution of m6A-circRNAs with a significant change in both the m6A level and circRNA expression level. B The distribution of correlation coefficients (Pearson correlation coefficient) between the m6A level and circRNA expression level. C Cumulative fraction of the circular ratio for m6A-circRNAs and non-m6A-circRNAs, respectively. The P value was calculated with the Wilcoxon rank sum test. D MeRIP-qPCR analysis of m6A levels of 7 indicated circRNAs in PDAC cell line with METTL3 knockdown and control. E Circulation index of 7 indicated circRNAs in PDAC cell line with METTL3 knockdown and control (bottom panel). Circulation index represent the relative abundance of each circRNA to its pre-mRNA. The abundance of circRNA and its pre-mRNA were determined using qRT-PCR. Top panel showed the scheme of primer design for circRNA and linear RNA to calculate circularization index. “C” and “L” primer sets are used to quantify circRNAs and linear RNAs. Data in D and E were means ± S.E.M. (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 of Student’s t test