Fig. 1
From: Wnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation
Identification of Wnt-regulated lncRNAs from orthotopic RNF43-mutant pancreatic cancer model. a Computational pipeline to identify 1503 Wnt-regulated lncRNAs from orthotopic RNF43-mutant pancreatic cancer. b Comparison of Wnt-regulated lncRNAs with Ensembl build 79 and FANTOM5 lncRNA annotations. c Expression profiles of 1503 Wnt-regulated lncRNAs across time points after Wnt inhibition. d Gene expression of selected Wnt-regulated lncRNAs, including annotated lncRNAs (VPS9D1-AS1 and ABHD11-AS1) and novel lncRNAs (XLOC_017401 and XLOC_045229). TPM, transcripts per million. e–g Fold change of lncRNAs after Wnt inhibition compared across models. More lncRNAs respond to Wnt inhibition in the HPAF-II subcutaneous (e) and orthotopic models (f) than in HPAF-II cells cultured in vitro. FC, fold change. g More lncRNAs respond to Wnt inhibition in HPAF-II orthotopic model than in the subcutaneous model