Fig. 9

LINC00301 facilitates proliferation, migration, and invasion through regulation of EAF2/VHL/HIF1α pathway in NSCLC cells. a Represent images for EAF2, pVHL, HIF1α in LINC00301 KD, and LINC00301 OE A549 and SPC-A-1 cells analyzed by immunofluorescence. b IB tested EAF2, pVHL, HIF1α levels in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2 transfected A549, and SPC-A-1 cells. c IB tested EAF2, pVHL, HIF1α levels in pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 transfected in A549, and SPC-A-1 cells. d Colony formation assays (seeded in 6-well plate) tested cell proliferation in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. e Trypan blue staining tested cell viability in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. g Transwell migration assay tested migration activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. f Transwell migration assay tested migration activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. g Transwell invasion assay tested invasive activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. Assays were conducted in triplicate. *p < 0.05, means ± SD was shown. Statistical analysis was performed by Student’s t-test