Fig. 4
From: CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
CRISPR-SONIC enables combinatorial Kras knockin and p53 knockout in B6 wildtype mice. a Schematic showing the target genomic locus, guide RNAs, and donor plasmid for in vivo integration. b Quantification of liver tumor per mouse. Error bars are s.d. (n = 5 mice). c Representative H&E and IHC staining detected GFP-positive and Ck19-positive tumor cells in +sgActin mice. Arrows denote internal control Ck19 staining in bile ducts. Scale bars are 75 μm