Fig. 3
From: CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
CRISPR-SONIC enables IRES-HRASG12V integration in vivo for tumor modeling. a Schematic showing the target genomic locus, guide RNAs, and donor plasmid for in vivo integration. b Western blot confirms HRAS overexpression resulting from targeted integration of Neuro2A cells 5 days post transfection. c Liver tumors from injected mice harvested at 1 month. d Number of surface liver tumors. Error bars are s.d. (n = 3 mice). e PCR detected integration band in liver tumor genomic DNA. T1-T3 are tumors from three mice (n = 3). f Liver tumors are positive for the ICC marker Ck19. Scale bars are 25 μm