Table 3 TP53 Mutations in Study Cohort

LTS-001

LT

Nonsense

p.S183*

0.47

no

Mutect

LTS-002

ST

Missense

p.E224D

0.27

no

Mutect

LTS-003

ST

Missense

p.R175H

0.83

Structural Change

yes

Mutect

LTS-004

LT

Frame Shift Del

p.P223fs

0.45

no

Strelka

LTS-005

ST

Missense

p.D281E

0.75

no

Mutect

LTS-006

ST

Missense

p.Y220C

0.46

Structural Change

yes

Mutect

LTS-007

LT

Missense

p.I195T

0.15

no

Strelka SNV/None by targeted seq

LTS-008

ST

Missense

p.C242F

0.64

no

Mutect

LTS-009

ST

Missense

p.M237I

0.57

no

Mutect

LTS-010

ST

Missense

p.Y220C

0.89

Structural Change

yes

Mutect

LTS-011

LT

Missense

p.R248Q

0.51

Structural Change

yes

Mutect

LTS-012

LT

Missense

p.R248Q

0.76

Structural Change

yes

Mutect

LTS-013

LT

Frame Shift Del

p.A70fs

0.57

no

Varscan2/Targeted Sequencing

LTS-014

LT

Splice Site

c.e7+1

0.89

no

Strelka SNV/Targeted Sequencing (g.chr17:7577498C > A)

LTS-015

ST

Splice Site

c.e8+1

0.74

no

Mutect/Strelka SNV

LTS-016

LT

Missense

p.R248Q

0.82

Structural Change

yes

Mutect

LTS-017

LT

Missense

p.I195T

0.7

no

Mutect

LTS-018

ST

Missense

p.G266E

0.73

no

Mutect

LTS-019

LT

Missense/Frame shift Ins

p.K139Q/ p.V143fs

0.72

no

Mutect/Strelka

LTS-020

LT

Splice Site

p.Q331Q

0.62

no

Mutect

LTS-021

LT

Missense

p.R248W

0.39

DNA binding

yes

Mutect

LTS-022

LT

Missense

p.G245S

0.72

Structural Change

no

Mutect

LTS-023

LT

Missense

p.T125P

1

no

Exome & Targeted sequencing

LTS-024

ST

Missense

p.R282W

0.6

Structural Change

no

Mutect

LTS-025

LT

Missense

p.R273H

0.91

DNA binding

yes

Targeted Sequencing

LTS-026

ST

Nonsense

p.E349*

0.46

no

Mutect

LTS-027

LT

Nonsense

p.R196*

0.56

no

Mutect

LTS-028

ST

Nonsense

p.G266*

0.93

no

Mutect

LTS-029

LT

Missense

p.Y163H

0.73

no

Mutect

LTS-030

LT

Missense

p.R273C

0.67

DNA binding

yes

Mutect

LTS-031

LT

Not detected

Not detected

–

no

None detected by WES on all callers/poor RNAseq

LTS-032

LT

Nonsense

p.W146*

0.86

no

Mutect

LTS-033

ST

Missense

p.R175H

0.4

Structural Change

yes

Mutect

LTS-034

ST

Missense

p.R273L

0.8

yes

Also found in normal (transformed adjacent normal)

LTS-035

ST

In Frame Insertion

p.266_267insLG

0.18

DNA binding

no

Strelka Exome & RNAseq

LTS-037

ST

Frame Shift Del

p.P87fs

0.77

no

Strelka

LTS-038

ST

Missense

p.R175H

0.63

Structural Change

yes

Mutect

LTS-039

ST

Missense

p.F270S

0.68

no

Strelka SNV

LTS-040

ST

Nonsense

p.E204*

0.51

no

Mutect