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Fig. 2 | Genome Medicine

Fig. 2

From: Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq)

Fig. 2

Assessments of the RM-seq protocol. a Error correction evaluation. RM-seq error correction combining merging of paired-end reads with consensus sequence determination from grouped reads sharing identical barcode allows a three order of magnitude reduction in false SNP calling when compared with raw reads calling for the different base. b Quantification of populations of S. aureus rpoB mutants. Three independent assessments of rpoB mutants from three independent genomic DNA preparations originating from a defined population are presented by the different blue bars (technical replicates). c Correlation of observed versus expected SNV frequencies. Blue points represent means and error bars represent SEM of three technical replicates. The blue line represents the linear regression of the frequencies measured by RM-seq and the dashed line represent the perfect correlation between expected and observed frequencies. d Quantification of S. aureus rpoB mutants from a complex population of in vitro selected rifampicin-resistant mutants. Columns represent mean normalised counts of the different rpoB mutations that were observed among all triplicates, and error bars represent SEM

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