Fig. 1
ICG-001 inhibits growth of OSCC cell lines and promotes an epithelial phenotype. a Immunofluorescence imaging of β-catenin localization in OSCC cell lines in response to ICG-001 treatment showing more focused membrane localization of β-catenin, increased intercellular adhesion with a more pronounced epithelial phenotype. Size bars, 10 μm. b ICG-001 treatment promotes cell compaction as measured by the average cell size (n = 15) captured in 1a. c Increased junctional organization of β-catenin in immunofluorescence images shown in 1a was determined based on the thickness of β-catenin at the membrane (n = 10). d Determination of half maximal effective concentration (EC50) for ICG-001 effects in OSCC cell lines. Shown are graded dose response curves for ICG-001 in CAL27, HSC-3, SCC9, and SCC25 cell lines for 72 h of treatment. Immunofluorescence images were quantified using ImageJ and compared using PRISM GraphPad. **P < 0.001; ***P < 0.0001 unpaired t test