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Fig. 7 | Genome Medicine

Fig. 7

From: BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data

Fig. 7

Ig transcript reconstruction in rhesus macaques with poor immunoglobulin reference annotation. a Pipeline for Ig assembly using unfiltered and filtered approaches (Filter-Non-IG: Discard reads mapping to non-Ig annotated regions of rhesus genome; IG_mapped: select reads mapped to the Ig coordinates and IG_mapped+Unmapped: combine IG_mapped reads and Unmapped reads for assembly). Ig reconstruction was carried out for 42 plasmablasts, 33 memory B cells, and 33 germinal center (GC) B cells. b Concordance of V(D)J gene annotation and CDR3 nucleotide sequence of Filter-Non-IG method with nested RT-PCR sequences from plasmablast and GC B cells

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Genome Medicine

ISSN: 1756-994X

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