Fig. 3

Gene ontology and signalling pathway analysis of the transcriptomic changes induced by RA and MYCN overexpression. a GO disease and function terms associated with neuronal differentiation, generated by analysing the SY5Y-MYCN RNA-seq data with IPA. GO term activation/inhibition levels are relative to those of the SY5Y-MYCN control cells. Full x-axis labels for the first, fourth and tenth bars, respectively, are: ‘differentiation of tumour cell lines’, ‘formation of plasma membrane projections’ and ‘differentiation of neuroblastoma cell lines’. b Top 15 differentially expressed signalling pathways (ranked by p value of overlap) between the 24-h 1-μM RA and 24-h 1-μl/ml DMSO vehicle control SY5Y-MYCN RNA-seq treatments, as generated by IPA analysis. c Retinoic acid receptor (RAR) signalling pathway overlaid with genes differentially expressed upon 24-h 1-μM RA treatment, as detected by RNA-seq. Pathway generated using IPA. Red shaded genes are upregulated by RA compared to control cells, while green ones are downregulated by RA. Intensity of shading indicates the degree of up- or downregulation, with deeper colours associated with higher levels of differential expression