Fig. 4

Altered gut microbiota community structure persists after correction of chronic dietary restriction. a–d PCA on days 69 (a), 96–97 (b), 98–99 (c), and 120 (d). The first two PCs from the PCA on unweighted UniFrac distance matrix are plotted. Symbols and colors represent data from individual diet regimens. The main axes of the ellipses correspond to the first two PCs with the height and width representing variances in the corresponding coordinates. Note that the LF-RF and TLF-RF data remained different from the AL data on day 120 despite a significant separation from the LF group; and that IGF1 treatment (TLF-RF group) mitigated the difference from the AL mice