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Fig. 1 | Genome Medicine

Fig. 1

From: Epigenomic and metabolic responses of hypothalamic POMC neurons to gestational nicotine exposure in adult offspring

Fig. 1

Ad libitum nicotine intake led to high plasma cotinine levels, moderately reduced fluid intake, and did not alter body weight or food consumption of dams. a Twenty-four-hour fluid intake was determined in group-housed females (n = 4 cages of 3 females/group) before pregnancy and in individually housed females (n = 10/group) during gestation. b Nicotine intake before (n = 4) and during pregnancy (n = 10) was calculated based on 24-h fluid intake. c Plasma cotinine levels were determined by enzyme-linked immunosorbent assay (ELISA) after 4 weeks of nicotine ingestion. Plasma cotinine was detected in all nicotine-exposed females (n = 6) and was undetectable in non-exposed females (n = 6). d Twenty-four-hour food intake was determined in group-housed females (n = 4 cages of 3 females/group) before pregnancy. e Body weight was determined weekly before pregnancy (n = 12/group). Data were analyzed by two-way RM ANOVA followed by Bonferroni post-test (a) or by Mann–Whitney test (c) (**, p < 0.01). All data are expressed as mean ± SEM

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