Fig. 2

AbR response to TIV across patients. Different metrics were measured for each patient and at each time-point. Metrics are delineated by row, and patients are delineated by column. a Overall Ab expression for each patient/time-point. b CDR3 diversity for each patient/time-point. B cells and PBMCs are shown in red and blue, respectively. c, d Stacked area charts showing the gene expression level for each IGHV gene for each patient/time-point. Colors, corresponding to IGHV genes, are comparable between patients and sample types, and were sorted by absolute range (max–min). c B cell. d PBMC data. Complete definitions for the y-axis units in (a, b, c, and d) can be found in methods. e ELISA results giving the concentration of Abs that bind TIV for the A, M, and G Ab isotypes (red, blue, and green, respectively). f Hemagglutinin inhibition assay results for the three different virus strains in the administered TIV, A/C: A/California/7/2009; B/B: B/Brisbane/60/2008; A/P: A/Perth/16/2009. Data for (e) and (f) were generated by [30], and downloaded from ImmPort [51], [ImmPort:SDY224]. *Patient 2 PBMC data at day 8 were unavailable due to sample processing error [30]