Skip to main content
Fig. 1 | Genome Medicine

Fig. 1

From: Profiling tissue-resident T cell repertoires by RNA sequencing

Fig. 1

Schematic representation of T cell receptor sequencing (TCR-seq) versus RNA sequencing. Horizontal lines represent mRNA transcripts with gray poly-A tails. Each color represents a unique gene sequence. a A pool of all mRNA in a sample is depicted, which contains irrelevant transcripts (blue, brown, and red) as well as recombined TCR transcripts (multi-colored). b TCR-seq involves selective amplification of the CDR3 region of TCR transcripts (displayed as a color gradient) by reverse transcription polymerase chain reaction (PCR) shown using a conserved C-gene primer (purple with black sequencing adapter tails) for the initial reverse transcription step and resulting, after PCR (not shown), in an enriched set of recombined TCR sequences. c RNA-seq employs shotgun sequencing, generating fragments from all transcripts present in the sample, which then have sequencing adapters ligated (black). The resulting sequencing library will contain fragments that, by chance, contain the CDR3 encoding sequence. Additionally, these libraries may contain fragments that share sequence similarity to recombined TCR sequences (e.g., the red transcript), potentially leading to false-positives

Back to article page