Fig. 2

Identification of DMPs associated with aGVHD severity. a Genomic locus on chromosome 14q24.2 harboring four top-ranked DMPs associated with aGVHD severity. A DNA hypomethylation phenotype was observed in HSCT donors matched to recipients with severe aGVHD (red points) compared to donors paired with recipients with no/mild aGVHD (blue points). Lines represent the means of the measured DNA methylation levels (β-values) across HSCT donors. Statistically significant DMPs are indicated with a black triangle. Annotation of all significant DMPs is provided in Table 2. b ROC measures evaluating the epigenetic classifier performance. LOOCV was used to assess the classifier performance (Methods). Over 85 iterations of the LOOCV, the mean AUC was 0.98 (95 % confidence interval = 0.96–0.99), with a maximal specificity and sensitivity of 0.93 and 0.93, respectively