Fig. 2
From: Practical guidelines for B-cell receptor repertoire sequencing analysis
The essential steps in repertoire sequencing analysis. Repertoire sequencing (Rep-seq) analysis can be divided into three stages: pre-processing; inference of B-cell population structure; and detailed repertoire analysis. Pre-processing transforms the next-generation sequencing reads into error-corrected B-cell immunoglobulin receptor (BCR) sequences, which are then aligned to identify the V(D)J germline genes. Next, the dynamic population structure of the BCR repertoire is inferred. Finally, quantitative features of the B-cell repertoire are calculated. MID multiplex identifier, SHM somatic hypermutation