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Figure 3 | Genome Medicine

Figure 3

From: Haploinsufficiency of Hedgehog interacting protein causes increased emphysema induced by cigarette smoke through network rewiring

Figure 3

Increased lymphocyte activation in the lungs of Hhip +/- mice exposed to cigarette smoke (CS). (A) Representative Hematoxylin and Eosin staining images of lymphocyte aggregates around airways in murine lungs exposed to 6 months of air or CS. (B) Quantification of the number of lymphoid aggregates per airway (mean internal diameter of 200 to 1,000 μm) in (A) +/+: Hhip +/+ mice; +/-, Hhip +/- mice. Data are mean ± SEM from >8 mice per group. (C) Correlation of the number of lymphoid aggregates per airway (X-axis) with the airspace size (mean chord length, MCL, Y-axis) of Hhip +/- mice exposed to CS for 6 months. Pearson correlation coefficients (r) and statistical significance (p) of the correlation coefficient are shown in the figure. (D) Representative triple immunostaining for CD20, CD4, and CD8 in a lymphocyte aggregate in a lung section from a Hhip+/- mouse exposed to CS for 6 months. (E) Total leukocyte counts in bronchoalveolar lavage samples from Hhip +/+ and Hhip +/- mice exposed to CS for 2 months. Data are means ± SEM from 10 to 11 mice per group. (F) Percentage of the CD8 + CD69+ T cells among total CD8+ T cells in lungs after enzymatic digestion from Hhip +/- and Hhip +/+ mice exposed to CS for 2 months quantified using immunostaining and flow cytometry. Data are mean ± SEM; 3 to 6 mice per group. *P <0.05, **P <0.01.

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