Figure 2

Gene expression profiling in lungs from Hhip ^+/- and Hhip ^+/+ control mice exposed to either room air (Air) or cigarette smoke (CS) for 6 months. (A) Venn diagram shows comparisons of differentially expressed genes in Hhip ^+/+-CS vs. Hhip ^+/+-Air (yellow oval), Hhip ^+/+-CS vs. Hhip ^+/--CS (green oval), Hhip ^+/--Air vs. Hhip ^+/+-Air (red oval) and genes enriched based on interaction with smoke treatment (blue oval). (B) Real-time PCR showed differential gene expression of Gstp1 and also of a subset of genes (C) revealed by gene-by-treatment interaction analysis. Relative gene expression levels were expressed as fold changes after normalized to Gapdh (glyceraldehyde 3-phosphate dehydrogenase) as the reference gene. Mean ± SEM are shown; n = 6 mice/group. Unpaired t test. *P <0.05; **P <0.01. ***P <0.001.