Figure 4

Timothy syndrome-associated modules are regulated by known calcium-dependent transcription factors. The TS-associated modules are enriched for targets regulated by known calcium-dependent transcription factors, MEF2, NFAT, CREB, and FOXO, which are listed in the middle of the figure. Dashed lines indicate significant enrichment of the designated transcription factor binding targets (P <0.05) in the corresponding modules (blue lines indicate P <0.01). The top 20 connected genes in each module are plotted according to the multidimensional scaling of their pairwise co-expression correlations, so that co-expressed genes are clustered to each other. Genes are connected if their pairwise correlations are higher than 0.7. Pie chart: ChIP-seq verified FOXO targets (blue); ChIP-seq verified NFAT targets (red); ChIP-seq verified CREB targets (green); ChIP-seq verified MEF2 targets (yellow). Genes with multiple probes are labeled separately.