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Figure 11 | Genome Medicine

Figure 11

From: Human-specific epigenetic variation in the immunological Leukotriene B4 Receptor (LTB4R/BLT1) implicated in common inflammatory diseases

Figure 11

Expression and signaling of LTB4R (BLT1) in PBMCs isolated from human and rhesus macaque peripheral blood. (A) Real-time RT-PCR analysis of LTB4R mRNA in isolated human and rhesus macaque PBMCs normalized to the reference gene (18s); results from seven different human donors and from pooled rhesus macaque PBMCs run as four separate experiments; mean ± standard error of the mean. (B) Human and rhesus macaque PBMCs were stimulated with indicated concentrations of LTB4 and intracellular calcium mobilizations were recorded. Results are expressed as the ratio of stimulated over basal (S/B) peak calcium fluxes obtained from three different human donors and pooled rhesus macaque PBMCs run as four experiments analyzed simultaneously, mean ± standard error of the mean. (C) Human PBMCs were pre-incubated for 10 minutes with different concentrations of BLT1 inhibitor LY22398 and calcium mobilization was analyzed in response to LTB4 (300 nmol/L). Data are presented as mean ± standard error of the mean percentages of maximum response to LTB4 (N = 3).

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