Figure 4
Differentially methylated probes (DMPs) associated with preterm birth identified using birth and 18-year blood spots. (A) Multi-dimensional scaling (MDS) and (B) heatmap plots show 109 combined preterm DMPs (comparing preterm with term, combining birth and 18-year samples for both groups). (C) Scatterplot displaying mean methylation differences (δβ) of birth (x-axis) and 18-year (y-axis) samples. Volcano plots showing δβ (x-axis) versus nominal P-values (−log10 scale) of combined preterm DMPs, with red points denoting probes with absolute methylation difference of greater than 0.05 and nominal P < 0.01 at (D) 18 years and (E) birth. The sample groups are color coded as follows: term birth, blue; preterm birth, black; term birth at 18 years, green; preterm at 18 years, red.