Figure 3

Gene-gene interaction with Yor1 and Yor1-ΔF serves as a resource for identification of novel regulators of membrane protein biogenesis. Interaction scores for individual yeast mutants in the context of either wild type Yor1 (x-axis) or Yor1-ΔF (y-axis) were plotted to illustrate classes of protein biogenesis regulators suggested by the screen. Deletion enhancers (positive value indicates prolonged L and slower growth) and suppressors of oligomycin sensitivity represent factors predicted to promote or prohibit protein biogenesis, respectively. Interactions along the y-axis may indicate proteins that act more specifically on misfolded proteins, while those in the upper right and lower left quadrants are considered to have more general effects on Yor1 protein biogenesis, or to affect the phenotype independently of Yor1/Yor1-ΔF. Examples of genes with functions suspected to directly influence oligomycin resistance, without necessarily acting through Yor1 biogenesis are denoted by 'x'. EMC members are colored in burgundy. CUE1, RPN4, and ERV14 are other genes that were further validated by molecular studies (see Figures 4, 6, and 7).