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Table 1 Advances in the optimization of tissue culture conditions for clinical development of hESCs

From: Challenges to the clinical application of pluripotent stem cells: towards genomic and functional stability

Optimization of culture conditions of hESCs for clinical use

References

Elimination of xeno-components

Replacement of mouse feeder layer cells with human feeder layer cells

[18–21]

 

Replacement of mouse feeder layer cells with human extracellular matrix

[22–25]

 

hESC lines successfully banked under good laboratory practice conditions

[15–17]

Scaling up of production of hESCs

Identification of small molecules that promote hESC self-renewal

[26–29]

 

Synthetic microcarriers to support hESC culture in suspension

[29–32]