From: Transcriptomic analysis of pluripotent stem cells: insights into health and disease
Characteristic | Mouse iPSCs | Human iPSCs |
---|---|---|
mRNA expression | Distinct from mESCs at lower passages, donor cell gene expression still present [62, 69]; closely resemble mESCs at late passages [62] | Distinct from hESCs at lower passages [58], with residual donor gene expression [70, 96, 97]; closely resemble hESCs at late passages [58, 98] |
miRNA expression | miRNA encoded within the imprinted Dlk1-Dio3 locus is aberrantly silenced [60] | Small number of differences reported [58, 99], but variation between hESCs and hiPSCs comparable to somatic and cancer cells [100] |
lncRNA expression | Not determined | Differences in lincRNA expression reported. lincRNA-RoR enhances reprogramming by twofold [13] |
DNA methylation status | Distinct from mESCs at lower passages, donor cell DNA methylation pattern still present [62, 69]; closely identical to mESCs at late passages [62] | Differences in DNA methylation reported [59, 68, 70], but not in all hiPSCs [71] |
Genome status | Not determined | Possess gene copy number deletions and duplications [63, 64], somatic coding mutations [65], and chromosomal duplications [66] |