Figure 3
From: Future medical applications of single-cell sequencing in cancer
Single-nucleus sequencing of breast tumors. (a) Single-nucleus sequencing involves isolating nuclei, staining with 4',6-diamidino-2-phenyl indole dihydrochloride (DAPI), flow-sorting by total DNA content, whole genome amplification (WGA), Illumina library construction, and quantifying genomic copy number using sequence read depth. (b) Phylogenetic tree constructed from single-cell copy number profiles of a monogenomic breast tumor. (c) Phylogenetic tree constructed using single-cell copy number profiles from a polygenomic breast tumor, showing three clonal subpopulations of tumor cells.