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Figure 2 | Genome Medicine

Figure 2

From: Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes

Figure 2

miRNA expression profile changes in T2D compared with control subjects using the Exiqon chip platform and TaqMan confirmation (FDR <10%). (a) Data are plotted to show the pattern of change of these significantly up-/down-regulated miRNA. Black lines represent those miRNA that increase/decrease progressively with IGT and T2D (DM), green lines represent miRNAs that are increased/decreased with IGT and then revert with T2D, while orange lines show miRNAs increased/decreased only in the T2D state. (b) miRNAs that show the expression profile during myocyte differentiation (cell data derived from Chen et al. [55]) is the opposite pattern to that observed in the muscle of patients with T2D (green = down-regulated probe sets, red = up-regulated probe sets; the color range is from -3-fold to +3-fold change). MG refers to the data produced by Chen et al. during myogenesis. (c)Expression level of miR-1, miR-133a, miR-133b and miR-206 in muscle biopsies from healthy individuals (NGT, n = 10, white bars), individuals with impaired glucose tolerance (IGT, n = 10, grey bars) and individuals with type 2 diabetes (T2D, n = 10, black bars). miR-133a (P < 0.001) and miR-206 (P = 0.04) were significantly reduced in T2D patients when compared with expression levels in healthy controls. Data are expressed as fold change from NGT and shown as mean ± standard error. ** P < 0.001, * P < 0.05. (d) Expression level of miR-133a in muscle versus indices of glucose homeostasis in subjects with and without T2D. Expression of miR-133a is positively correlated with fasting glucose, R2 = 0.41 (P < 0.001, n = 30). Data are shown as ΔCt levels normalized to RNU48 and plotted versus fasting glucose levels (mmol/L).

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