Figure 3From: Using transcriptomics to identify and validate novel biomarkers of human skeletal muscle cancer cachexiaCAMkIIβ and TIE1 correlate with weight loss in cancer cachexia. In order to validate the findings from the rectus abdominis, qRT-PCR was used to examine mRNA expression of (a) CAMkIIβ and (b) TIE1 in diaphragm (open circles) and vastus lateralis (closed circles) in a separate clinical cohort. Correlation plots for mRNA level against rate of weight loss are shown. Correlation coefficients were significant with P < 0.05. CAMkII protein and phospho-protein levels are increased in subjects with weight loss. (c) Protein levels of CAMkII and (d) phosphoCAMkII were assessed in the rectus abdominis muscle from center 1 subjects by western blot. Intensity levels were normalized against alpha-skeletal actin and the mean ratio of CAMkII/actin or phosphoCAMkII (pCAMkII)/actin are shown for subjects with less than (black) or more than (white) 5% weight loss. *P-value < 0.05, one-sided Mann Whitney test; n = 59. Error bars represent SEM.Back to article page